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KMID : 0545120070170061018
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Journal of Microbiology and Biotechnology
2007 Volume.17 No. 6 p.1018 ~ p.1023
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Cloning of Fibrinolytic Enzyme Gene from Bacillus subtilis Isolated from Cheonggukjang and Its Expression in Protease-deficient Bacillus subtilis Strains
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Jeong Seon-Ju
Kwon Gun-Hee
Chun Ji-Yeon
Kim Jeong-Sang
Park Cheon-Seok
Kwon Dae-Young
Kim Jeong-Hwan
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Abstract
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Bacillus subtilis CH3-5 was isolated from cheonggukjang prepared according to traditional methods. CH3-5 secreted at least four different fibrinolytic proteases (63, 47, 29, and 20 kDa) into the culture medium. A fibrinolytic enzyme gene, aprE2, encoding a 29 kDa enzyme was cloned from the genomic DNA of CH3-5, and the DNA sequence determined. aprE2 was overexpressed in heterologous B. subtilis strains deficient in extracellular proteases using a E. coli-Bacillus shuttle vector. A 29 kDa AprE2 band was observed and AprE2 seemed to exhibit higher activities towards fibrin rather than casein.
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KEYWORD
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Cheonggukjang, fibrinolytic enzyme, Bacillus subtilis, gene expression
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